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The tested strains (ΔyhaK, ΔyhaJ and the wild type, BW25113) were cultured in TGA overnight and regrown as previously described. DNT was added to a concentration of 50 mg/L and the bacteria were incubated at 37°C, 200 rpm. Spent medium samples were collected at 0, 240, 300, 360, 420 and 480 min, and tested for the presence of the yqjF inducer in a luminescence assay as described above, using the yqjF::luxCDABE-harboring reporter strain.
Copyright and License information: ©2016 Palevsky, Shemer, Connolly and Belkin.
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