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NF-κB luciferase reporter assay
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RAW-ELAM macrophages were pre-treated with COR123625 (50 µM) for 1 h prior to stimulation with LPS (100 ng ml−1) for 4 h. Cells were lysed in 50 μl 1× passive Lysis Reporter Buffer (Promega). Luciferase activity was measured immediately after the addition of 30 μl of Luciferase Substrate Solution (Luciferase Assay System, Promega) using the Infinite M1000 Pro plate-reader (Tecan, Australia).
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