SNL model was performed according to our previous study (Wang W. et al., 2012). First, the rats were anesthetized with intraperitoneal injection of chloral hydrate (280 mg/kg). Next, the transverse process of the sixth left lumbar was cut off to expose the L4 and L5 spinal nerves. Subsequently, the L5 spinal nerve was separated and ligated by 6–0 silk sutures, and the incision was closed layer by layer. Rats in the sham group received the same procedures except for nerve ligation.
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