RNA extraction, DNAse treatments and quality control

RNA extractions were performed using the RNeasy Plant Mini Kit (Qiagen, Mississauga, Canada) as described by the manufacturer with the following modifications. Extractions were carried out using 100 mg of mycelium powder and the optional on-column DNase digestion with the RNase-Free DNase set was performed as described by the manufacturer. Each undiluted RNA extract was subjected to two consecutive rounds of DNAse treatment using a Turbo DNAse (TURBO DNA-free, Life Technologies, Carlsbad, CA, USA), as described by the manufacturer. Purified RNA was verified and quantified by using Experion StdSens chips (Bio-Rad, Mississauga, Canada) and stored at − 80 °C.