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Bacteria were incubated in the CK and G1 media at 37°C for 8 h. The cells were collected by centrifugation (4°C, 6000 × g, 5 min) and mixed with lyophilized protective medium (sterile 12% skim milk). After freezing at -20°C for 10 h, samples were freeze-dried at -55°C for 24 h. The freeze-drying products were then immediately suspended in sterile saline, and the activities of key glucose metabolism enzymes determined.
Copyright and License information: ©2018 Zhou, Jiang, Wang, Zhang and Zhao.
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