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Assessment of Intracellular Load of L. infantum

CT Clarissa R. Teixeira
CS Claire da S. Santos
DP Deboraci B. Prates
RS Rafael T. dos Santos
TA Théo Araújo-Santos
SS Sebastião M. de Souza-Neto
VB Valéria M. Borges
MB Manoel Barral-Netto
CB Cláudia I. Brodskyn
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Human neutrophils were treated or not with Lu. longipalpis SGS and infected with stationary-phase L. infantum (1 cell:5 parasites) for 3 h. After washing to remove non-internalized parasites, RPMI-1640 media was replaced for Schneider’s insect medium (Sigma Chemical Co., United States), supplemented with 10% heat-inactivated fetal bovine serum (Gibco, United States), L-glutamine (2 mM), penicillin (100 U/ml), streptomycin (100 mg/ml) (Invitrogen, United States) at 23°C for 24 h. Subsequently, the number of viable L. infantum were determined by counting live promastigotes using a Neubauer chamber.

Copyright and License information:  ©2018 Teixeira, Santos, Prates, dos Santos, Araújo-Santos, de Souza-Neto, Borges, Barral-Netto and Brodskyn.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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