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To inhibit STAT3 expression in U266 cells by RNA interference, U266 cells (2 × 106 cells/well) were transfected with 50 nM STAT3 siRNA (sc-29493; Santa Cruz Biotechnology) or 100 nM scrambled siRNA (SN-1002; BIONEER, Daejeon, Korea) using NEON Transfection system (Invitrogen). Then cells were incubated in 10% FBS-supplemented RPMI 1640 medium for 48 h.
Copyright and License information: ©2018 Jung, Lee, Nam, Narula, Namjoshi, Blough, Um, Sethi and Ahn.
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