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Hormone Replacement in Female Mice

KG Kathleen M. Gavin
TS Timothy M. Sullivan
WK Wendy M. Kohrt
SM Susan M. Majka
DK Dwight J. Klemm
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One to two weeks after BMT (at approximately 10–12 weeks of age, approximately 6 weeks after OVX), hormone replacement was initiated via implantation of subcutaneous pellets in those mice randomized to the E2 add-back group (Innovative Research of America, Estradiol: Cat. # SE-121, 0.05 mg/pellet, 60-day release) or a sham surgery control group. Pellets were implanted in the dorsal subcutaneous tissue of the mouse. One E2 pellet was introduced via 10-gauge trocar. Implants were expected to maintain circulating hormone levels in the physiological range with the doses previously utilized in other mouse studies of adipogenesis and E2 replacement after OVX (26).

Copyright and License information:  ©2018 Gavin, Sullivan, Kohrt, Majka and Klemm.
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