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Filtering 16S rRNA sequencing data for quality

HC Hui-Mei Chen
TC Tzu-Hao Chang
FL Feng-Mao Lin
CL Chao Liang
CC Chih-Min Chiu
TY Tzu-Ling Yang
TY Ting Yang
CH Chia-Yen Huang
YC Yeong-Nan Cheng
YC Yi-An Chang
PC Po-Ya Chang
SW Shun-Long Weng
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Sequencing reads from different samples were identified and separated according to specific barcodes at the 5′ of the sequence. The FASTX-Toolkit (http://hannonlab.cshl.edu/fastx_toolkit) was employed to process the raw read data files, and the reads were processed for sample identification and trimming barcode, adaptor, and low quality bases. The bases with quality lower than Phred quality score 20 were removed. Sequences consisting of less than 100 nucleotides were discarded along with any reads containing ambiguous characters.

Copyright and License information: The Author(s). ©2018
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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