SETD7 protein levels were quantified by Western blot analysis of whole cell extracts using the antibodies anti-SETD7 (1:1000, Proteintech Group, Cat# 24840–1-AP, RRID: AB_2737406) and anti-GAPDH (1:2000, Abcam, Cat# ab181602, RRID: AB_2630358). GAPDH served as a loading control.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.