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Cell apoptosis analysis

RG Rui Gao
XM Xuan Mei
DY Dongpeng Yan
RL Ruizheng Liang
MW Min Wei
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For the cell apoptosis analysis, Hela cells were seeded into 6-well plates in the density of 2 × 105 per well and incubated for 24 h. Then the culture medium was replaced by 2 mL of DMEM containing IPA or IPA/LDH (in the concentration of 2 μg mL−1) and cells were incubated at 37 °C. After 24 h, cells were washed thoroughly with PBS and irradiated under 808 nm laser. After incubation for another 6 h, cells were collected and stained using an Annexin V-FITC Apoptosis Detection Kit purchased from Solarbio Life Sciences (Beijing, China), and examined with flow cytometry.

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