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Microstructure by polarized light microscopy (PLM)

SG Saeed M. Ghazani
AM Alejandro G. Marangoni
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The microstructure was imaged by PLM using an optical microscope model BX60 (Olympus Optical Co., Tokyo, Japan) equipped with a 20× objective lens. Images were captured (20×) with a model DP71 digital camera (Olympus Optical Co., Tokyo, Japan) using the v1.0 cellSens software. A 5 μL sample of each molten fat was placed on a preheated (80 °C) glass microscope slide and covered with a preheated (80 °C) glass coverslip to remove excess fat and air pockets. Slides were heated at 80 °C for 15 minutes to erase the crystal memory, then cooled to 22 °C and held for one week prior to observations.

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