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Immunochemical analysis of protein level and phosphorylation was performed by Western blotting method in standard conditions. The sample was mixed with Laemmli buffer (2×) and denatured for 5 min at 95 °C. After standard SDS-PAGE on polyacrylamide gel, the proteins were transferred onto a nitrocellulose membrane and detected with specific antibodies. GAPDH level was analysed as a loading control. Densitometric analysis and size-marker-based verification was performed with TotalLab4 software.
Copyright and License information: The Author(s). ©2018
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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