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Detection of ROS Accumulation and Cell Death using Biochemical Staining

DZ Dandan Zang
HL Hongyan Li
HX Hongyun Xu
WZ Wenhui Zhang
YZ Yiming Zhang
XS Xinxin Shi
YW Yucheng Wang
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Detached leaves from different Arabidopsis lines were treated with 200 mM NaCl or 300 mM mannitol for 0, 6, and 12 h, and then used for histochemical staining analysis. The leaves were infiltrated with DAB or NBT, which allowed the detection of H2O2 and O2-, respectively, according to the method (Fryer et al., 2002). Evans Blue staining was performed to investigate the cell death, as described (Zhang L. et al., 2011). H2O2 was measured following the protocol of Thordal-Christensen et al. (1997).

Copyright and License information:  ©2016 Zang, Li, Xu, Zhang, Zhang, Shi and Wang.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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