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IC50 values for LSD1 activity after chemical inhibition were measured as previously described [25]. Briefly, this involves incubation of the purified enzyme with an H3K4me2 peptide substrate and measurement of by-product H2O2 using AmplexRed® (Invitrogen).
Copyright and License information: The Author(s) ©2018
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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