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Active CDK2/cyclin A (14–448) was purchased from Millipore. Kinase assays were performed in the presence of (γ-32P) ATP by using an in vitro kinase buffer system from Millipore. Briefly, recombinant CDK and cyclin complexes were incubated with various substrates at 30 °C for 40 min. The reaction samples were subjected SDS–PAGE and autoradiography.
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