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In silico analysis

FV Frank Vandenbussche
EM Elisabeth Mathijs
DL David Lefebvre
KC Kris De Clercq
SB Steven Van Borm
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Melting temperatures of non-tailed/tailed panFMDV-3D forward primers were calculated using the nearest-neighbour thermodynamics model as implemented in Visual OMP (DNA Software) [41]. Secondary structure analyses were performed in Visual OMP (DNA Software) by modelling the interactions between the panFMDV-3D forward primer, 5’-nuclease probe and target sequences. Interactions were calculated at a reaction temperature of 60°C with the following reaction conditions: 50 nM monovalent cation, 3.3 nM divalent cation, 1 μM of primer and 1 pM of target. All other parameters were kept at their default values.

Copyright and License information:  ©2016 Vandenbussche et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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