FAME analysis.

The fatty acid methyl ester (FAME) analysis was performed with cells growing on BBL Trypticase soy agar plates (Becton Dickinson) at 28°C ± 1°C for 24 h. The extraction of fatty acid methyl esters was performed according to the standard protocol of the Sherlock Microbial Identification System (54). Bacterial biomass was harvested from the third sector of growth on agar plates, where the cultures reached the late-exponential stage of growth according to the four-quadrant streak method (54). Cellular fatty acid extracts were analyzed with an Agilent 7890B gas chromatograph using the rapid Sherlock microbial identification system (MIS) (version 6.2B, MIDI database RTSBA 6.21; MIDI Inc.). The quantities of individual fatty acids are given as percentages of all named fatty acids. Strains CCM 8730^T and CCM 8731^T and the reference strains were tested three times to evaluate the reproducibility of the method.