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Determination of the chelation

MZ Marzieh Zargaran
ES Ebrahim Saadat
RD Rassoul Dinarvand
MS Mohammad Sharifzadeh
FD Farid Dorkoosh
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Fourier Transform Infrared (FT-IR) spectroscopy is a method which by the formation of the chelate and also the percentage of chelated amino acid would be determined.15,16 There were two significant peaks in the FT-IR spectrum of free alanine, which disappeared upon chelate formation. One of them is due to symmetric stretching of carboxyl group and appeared at 1409 cm-1. The other one is at 1595 cm-1and was assigned to asymmetric stretching of carboxyl group.16

In order to determine the percentage of iron chelate, the consecutive concentrations of ultra-pure alanine in potassium bromide were provided. Then all of the prepared samples were scanned by FT-IR spectrophotometer. By measuring the absorbance of the significant peak at 1409 cm-1 and comparing it with the absorbance of standard alanine at the same wave number, the percentage of the free alanine in the mixture is determined accordingly.

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