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Matrigel (BD Biosciences, CA, USA) was subcutaneously injected either alone or mixed with VEGF (500 ng/mL) in a total volume of 500 μL into the ventral flanks of 7–8-week-old male null mice. Thereafter, these animals were treated with a daily dose of 50 mg/kg of JQ1 by intraperitoneal injection for 10 d. On day 11, the Matrigel plugs were removed and the Matrigel sections were stained with either H&E or Masson’s trichrome, or immunohistochemistry was performed using CD34 rabbit polyclonal antibody (Abcam, Cambridge, MA, USA).
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