Paraffin-embedded human NSCLC specimens used in this study were histopathologically diagnosed at the Sun Yat-sen University Cancer Center (SYSUCC). Clinical NSCLC specimens and the paired adjacent noncancerous lung specimens were frozen and stored in liquid nitrogen until further use. Adjacent non-tumor specimens were obtained from a standard distance (3 cm) from resected neoplastic tissues of NSCLC patients who underwent surgical lung resection and confirmed by pathological evaluation. For the use of these clinical materials for research purposes, prior patients’ consents and approval from the Institutional Research Ethics Committee were acquired. Clinical information of a cohort of 160 cases of NSCLC specimens are presented in Supplementary Table S3.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.