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Syringe infiltration of N. benthamiana leaves

VK Vaiva Kazanavičiūtė
AM Audrius Misiūnas
YG Yuri Gleba
AG Anatoli Giritch
AR Aušra Ražanskienė
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Plant agroinfiltration was performed as previously described21. A mix of three A. tumefaciens strains containing the 5′ provector, 3′ provector and integrase plasmid constructs was infiltrated into the abaxial side of the leaf using a syringe without a needle. MagnICON 5′ provector module for cytosol targeting (pICH20111) as well as the integrase expression vector pICH14011 were used together with 3´modules pNMDV503 and pNMDV516. Strains containing 5′ and 3′provector modules were used at dilution 1:100, and the integrase cassette - containing strain at dilution 1:40. Plant leaves were observed and collected at 5 to 6 days post infiltration. pICH20111 (5′ provector for cytosol expression) and pICH14011 (integrase) constructs are described in32.

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