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Western blot and immunoprecipitation

SJ Seung Hee Jung
HH Hyun Jung Hwang
DK Donghee Kang
HP Hyun A. Park
HL Hyung Chul Lee
DJ Daecheol Jeong
KL Keunwook Lee
HP Heon Joo Park
YK Young-Gyu Ko
JL Jae-Seon Lee
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Western blot (WB) and immunoprecipitation (IP) analyses were performed as described previously [22]. The following antibodies were used in this study: Primary antibodies included phospho-AKT at S473 (#4060), phospho-AKT at T308 (#13038), phospho-Foxo3α at Thr32 9 (#9464), phospho-AMPKα1 at Thr172 (#2535), phospho-p70S6K at Thr389 (#9206), phospho-SIN1 at Thr86 (#14716S), phospho-NDRG1 at Thr346 (#5482), phospho-p53 at Ser6 (#9285), Ser9 (#9288), Ser15 (#9284), Ser20 (#9287), and Ser37 (#9289), phospho-pRb at Ser807/811 (#9308), mTOR (#2972), Raptor (#2280), Rictor (#2140), TSC2 (#6935), 4EBP1 (#9452), and phospho-4EBP1 at Thr37/46 (#2855) (Cell Signaling Technology, Danvers, MA, USA); PTEN (#SC-7974), MDM2 (#SC-965), phospho-PKCα at Ser657 (#SC-12356), and p21 (#SC-397) (SantaCruz, Dallas, TX, USA); p53 (#NCL-L-p53-DO7) (DO7, Leica, Milton Keynes, UK); Actin (#G043) (Applied Biological Materials Inc.); Flag (#A8592), and Catalase (#C-0979) (Sigma); Myc (#OP10) (Calbiochem, CA, USA).

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