Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
The LV tissue was collected and total RNAs were extracted by Trizol following manufacture's protocol. The total RNAs were treat with DNAse Turbo to remove genomic DNA. RNA quality was assessed using Agilent Bioanalyzer Nano RNA Chip. 1 μg of total RNA (RIN > 8) was used to prepare the sequencing library using NEBNext Stranded RNA Kit with mRNA selection module. The library was sequenced on illumina HiSeq 4000 (single end, 50 base pair) at the Sequencing Core of University of Michigan.
Copyright and License information: ©2018 The Authors
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
