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Cell lysis was accomplished by freeze-thaw cycles in PBS and sonication. Proteins were cleaned by centrifugation (12,000×g). Superoxide dismutase (SOD) activity was performed following manufacturer's instructions (Sigma-Aldrich Quimica SL, Madrid, Spain). Samples were treated with 5 mM potassium cyanide 30 min before the assay to inhibit the activity of cytosolic CuZnSOD (SOD1) in order to quantify mitochondrial SOD2 activity. Protein concentration was quantified by using Bradford Assay for standardization (Bio-Rad Laboratories Inc, Madrid, Spain).
Copyright and License information: ©2018 The Authors
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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