Skin fibroblasts from PD patients and unaffected controls

KT Kenneth K. B. Tan
WL Wallie Wee Meng Lim
CC Chou Chai
MK Marek Kukumberg
KL Kah Leong Lim
EG Eyleen L. K. Goh
EY Evelyn K. F. Yim
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Skin fibroblasts from a 57 year old male PD patient carrying a LRRK2 G2019S mutation (ND29542), and age- and sex-matched unaffected control (AG04148; 56 year old male) were purchased from the Coriell Institute for Biomedical Research (NJ, USA). Fibroblasts were maintained in T-25 flasks in MEMα supplemented with 15% FBS (both from Life Technologies). Subculturing of cells was performed using 1× trypsin/EDTA solution (Sigma T-4174) with standard techniques.

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