Skin fibroblasts from a 57 year old male PD patient carrying a LRRK2 G2019S mutation (ND29542), and age- and sex-matched unaffected control (AG04148; 56 year old male) were purchased from the Coriell Institute for Biomedical Research (NJ, USA). Fibroblasts were maintained in T-25 flasks in MEMα supplemented with 15% FBS (both from Life Technologies). Subculturing of cells was performed using 1× trypsin/EDTA solution (Sigma T-4174) with standard techniques.
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