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HeLa cell preparation

SS Seungwoo Shin
DK Doyeon Kim
KK Kyoohyun Kim
YP YongKeun Park
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HeLa cells, a human cervical cancer cell line, were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Welgene) with 10% fetal bovine serum (FBS) (Welgene) and 1% penicillin streptomycin [100× (v/v), Welgene] inside a humidified incubator (37 °C, 5% CO2, 95% air). After 12 h of incubation, trypsin EDTA (Welgene) was applied to detach the cells from the culture flask. The detached cells were then collected via centrifugation (1000 rpm for 3 min) and incubated on a coverslip using the same previous culture conditions for 3 h. The diluted red fluorescent PS beads (100× diluted from stock solution) were added to the culture medium and became inserted as fluorescent probes inside the HeLa cells. Cells on the coverslip were maintained with the bead solution for 12 h and then washed three times with PBS solution.

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