Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Measurement of intracellular potassium ion

WJ Wenjing Jiang
GL Guangxin Li
WL Weidong Li
PW Ping Wang
PX Peng Xiu
XJ Xian Jiang
BL Bing Liu
XS Xueying Sun
HJ Hongchi Jiang
request Request a Protocol
ask Ask a question
Favorite

The methods have been described previously13. Briefly, PBFI-AM (Potassium-binding benzofuran isophthalate-AM) (Invitrogen™ Molecular Probes™, Thermo Fisher Scientific), a cell permeant potassium indicator, was used for measuring intracellular K+ content. After cultured cells were washed with Hank’s balanced salt solution (HBSS), 5 μM PBFI and 10 μM F-127 were added to cells, which were incubated for 40 min at 5% CO2 and 37 °C in the dark. Cells were washed again with HBSS thrice, and then subjected to a fluorescent microscope (Leica DMIRB, Germany). Measurements were made by exciting PBFI at 340 nm and monitoring emission at 500 nm. The fluorescence intensity was assessed by using the NIH imaging software Image J.

Copyright and License information: The Author(s) ©2018
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A