Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
In Vitro Stimulation of Bone Marrow-Derived Macrophages (BMDMs)
Request a Protocol
Ask a
question
Favorite
Bone marrow cells were isolated from femur and tibia of C57/BL6 mice and were cultured in L929 fibroblast conditioned RPMI 1640 medium (containing 15% L929 supernatant and 10% FCS) for 7 days. The resulting macrophages were stimulated over night with 20 MOI of live Hh. Cells were pre-incubated with DATS (10 µM) 30 min before the stimulation with Hh. Then, the supernatants were collected for the detection of IL-6 and TNFα cytokines by ELISA.
Copyright and License information: ©2018 De Cicco, Sanders, Cirino, Maloy and Ianaro.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
