Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Coomassie blue and Ponceau S staining

XW Xixi Wang
YH Yu He
YY Yang Ye
XZ Xinyu Zhao
SD Shi Deng
GH Gu He
HZ Hongxia Zhu
NX Ningzhi Xu
SL Shufang Liang
request Request a Protocol
ask Ask a question
Favorite

The SDS-PAGE gel was fixed in solution (50% methanol and 10% glacial acetic acid) for 2 hours with gentle agitation. It was stained in staining solution (0.1% Coomassie Brilliant blue R-250, 50% methanol and 10% glacial acetic acid) for 2–3 hours with gentle agitation. After destaining in solution (40% methanol and 10% glacial acetic acid) for several times until no unspecific background, the gel was stored in 5% glacial acetic acid.

The blotted membrane was immersed in a sufficient amount of Ponceau S staining solution (0.1% (x/v) Ponceau S in 1% (v/v) acetic acid) to stain for 5 minutes. Then membrane was immersed in an aqueous solution containing 5% acetic acid (v/v) for 5 minutes to evaluate the efficiency of protein transfer from the SDS gel to PVDF membrane.

Copyright and License information: The Author(s) ©2018
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A