Acute thrombosis induced by a combination of collagen and epinephrine in mice

Male C57BL/6 mice were fasted overnight and divided into groups of 10 animals. Each compound suspended in DMSO was administered to mice intravenously. A mixture of collagen (500 μg/kg) plus epinephrine (50 μg/kg) was injected to the tail vein of mice to induce acute thrombosis 1 h later. Each mouse was carefully examined for 15 min to determine whether the mouse was paralyzed, dead, or recovered from the acute thrombotic challenge. For statistical analysis, five separated experiments were performed.