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Acute toxicity experiment

XS Xinling Song
ZL Zhonghai Liu
JZ Jianjun Zhang
QY Qihang Yang
ZR Zhenzhen Ren
CZ Chen Zhang
ML Min Liu
ZG Zheng Gao
HZ Huajie Zhao
LJ Le Jia
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The acute toxicity experiment was processed using the method reported by Zhang et al.29. Twenty Kunming strain mice (male, 20 ± 2 g, 8-week-old) were obtained from Taibang Biologic Products Limited Company (Tai’an, China) and were randomly divided into two groups, with ten mice in each group. The mice in the control group received saline solution, while the mice in the experimental groups (EPS) orally received EPS at a dose of 4,000 mg/kg, and all the mice were fed by intragastric administration (i.g.). The mice were observed continuously for their appearance parameters and for any mortality or behavioural changes (respiratory distress, abnormal locomotion, or catalepsy) during the entire feeding period (14 consecutive days)39.

Copyright and License information: The Author(s) ©2018
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