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Determination of apoptosis by TUNEL analysis
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Terminal transferase-mediated dUTP nick-end labeling (TUNEL) staining was performed to assess apoptosis following hypoxic insult using the DeadEnd TM fluorometric TUNEL system kit (Promega, Madison, WI, USA) according to manufacturer’s protocol. In brief, 5-μm-thick paraffin-embedded brain tissue sections were subjected to analysis. The TUNEL-positive cells were visualized and analyzed by NIS-Elements BR imaging processing and analysis software (version 4.0, Nikon Corporation, Japan).
Copyright and License information: Med Sci Monit, ©2018
This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
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