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To test whether it would be possible to make large stocks of endotoxin-free protein powder for future use without the silk losing its mechanical properties, two processes were tested on dopes that had been autoclaved three times: (1) the autoclaved dope was centrifuged at 4,185 × g, the supernatant was removed, and the protein pellet was frozen; (2) the entire autoclaved dope was frozen. The frozen samples were then lyophilized, after which the protein was resolubilized in water. Films were then made using the standard procedure outlined above.
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