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Aortas were excised, cleaned of adventitia, and stained for atherosclerotic lesions using 0.5% oil red O stain prepared in 60% isopropanol. The images were photographed using a DS-Fil camera (Nikon) on a multizoom AZ100 microscope (Nikon), and the plaque burden was analyzed using Nikon Elements software and expressed as a percentage of total aortic area.
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