C. elegans survival assay

C. elegans survival analysis was performed as previously described^13,29. C. albicans was seeded on plates containing brain heart infusion (BHI) and kanamycin (45 μg/mL). Age-synchronous populations of young adults were washed from NGM plates containing their food source (E. coli. OP50) with M9 buffer, and added to the center of the C. albicans lawns. In the assay plates, 75 μg/mL of fluoro-29-deoxyuridine (FUdR) was added to prevent the growth of progeny. Infection was performed for 4 h at 25 °C after adding 60 animals to the plate. Next, animals were transferred into a single well of a tissue culture plate (Corning, Inc) containing 2 mL of liquid medium (80% M9, and 20% BHI) and kanamycin (45 μg/mL). Nematodes were scored as dead or live every 24 h. Nematodes would be scored as dead if no response was detected after prodding with a platinum wire. Three replicates were analyzed for each experiment.