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Fluorescence lifetime imaging microscopy (FLIM)
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Cells were seeded in Cell Carrier 384-Ultra plates (6057308, Perkin Elmer) for imaging. Fluorescence lifetime images were taken as described [41]. Briefly, we used an ISS-Alba FLIM/FCS confocal microscope with a 60x water immersion objective (NA = 1.2) and a PDL 800-D pulsed laser (PicoQuant) at a repetition rate of 20MHz. We used a 445/505/588nm excitation filter and a 628/30 nm emission filter. VistaVision Software was used for acquisition and analysis of the FLIM data.
Copyright and License information: ©2018 Canty et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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