2.4. MicroRNA Experiments

For the miRNA measurement, total RNA was obtained using a miRNeasy Mini Kit (Cat # 217004,QIAGEN, GmbH, Germany) following the manufacturer's instructions and subsequently checked for a RNA integrity number (RIN) to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US). The detailed methods for the miRNA experiments were described previously (Yu et al., 2017). We deposited the raw and processed data at the National Center for Biotechnology Information Gene Expression Omnibus (repository number {"type":"entrez-geo","attrs":{"text":"GSE103431","term_id":"103431"}}GSE103431).