Cell culture and chemicals

XY Xinyi Yu
YX Yunfeng Xia
LZ Liyi Zeng
XZ Xi Zhang
LC Liqun Chen
SY Shujuan Yan
RZ Ruyi Zhang
CZ Chen Zhao
ZZ Zongyue Zeng
YS Yi Shu
SH Shifeng Huang
JL Jiayan Lei
CY Chengfu Yuan
LZ Linghuan Zhang
YF Yixiao Feng
WL Wei Liu
BH Bo Huang
BZ Bo Zhang
WL Wenping Luo
XW Xi Wang
HZ Hongmei Zhang
RH Rex C. Haydon
HL Hue H. Luu
TH Tong-Chuan He
HG Hua Gan
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Mouse fibroblast line NIH-3T3 cells were obtained from ATCC (Manassas, VA) and maintained in complete Dulbecco’s Modified Eagle Medium(DMEM) containing 10% fetal bovine serum (Invitrogen, Carlsbad, CA), 100 units of penicillin and 100 µg of streptomycin at 37 °C in 5% CO2. Ang II was purchased from Sigma-Aldrich (St. Louis, MO). PI3Kγ inhibitor AS605240 was purchased from Selleckchem (Huston, TX). Akt and p-Akt antibodies were obtained from Santa Cruz Biotechnology (Dallas, TX); while Col1a, fibronectin, α-Sma and Gapdh antibodies were purchased from Abcam (Cambridge, MA). Unless otherwise indicated, all chemicals were purchased from Sigma-Aldrich (St. Louis, MO) or Thermo-Fisher Scientific (Pittsburgh, PA).

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