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A stock solution of 20% iodixanol was prepared from 1 ml OptiPrep (contains 60% iodixanol) by addition of 2 × 1 ml of DPBS supplemented with 2 % fetal bovine serum (FBS) by reversed pipetting.
A stock solution containing 36 % iodixanol was prepared in the same manner by adding 3 × 1 ml OptiPrep and 2 × 1 ml DPBS with 2 % FBS, using reversed pipetting. 3 μg ml−1 fluorescent tracer molecules, Dextran Cascade Blue 3000 Da, was added.
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