Wild-type Arabidopsis seeds (Columbia-0 ecotype) were purchased from Lehle seeds (Rock Round, TX). DR5::GUS seedlings of A. thaliana were provided by P. Masson (UW–Madison). The TIR1/IAA7 yeast two hybrid (Y2H) system was provided by M. Estelle (UC San Diego). Native and non-native AHLs were utilized from pre-existing stocks that were synthesized according to published protocols13,31,44,46. Synthetic auxins 20 and 21 were prepared using standard amide coupling protocols and yielded NMR and MS characterization data as expected53. Unless otherwise stated, all chemicals were purchased from Sigma Aldrich (St. Louis, MO).
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