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Cells were harvested at each indicated time point of 24, 48, and 72 h, washed twice with cold PBS, and stained with AnnexinV-APC and PI using APC-AnnexinV Binding apoptosis assay kit (Cat Nr. 22837, AAT Bioquest, USA) for 15 min at room temperature according to the manufacturer’s instructions (Invitrogen, China). Apoptosis cells were quantified using FACSCalibur™ cytometer (BD Biosciences, USA), and analyzed by CellQuestPro software.
Copyright and License information: The Author(s) ©2018
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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