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2.6. Tartrate-Resistant Acid Phosphatase (TRAP) Staining
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RAW264.7 cells were seeded in 24 well plates at a density of 2×104 cells/well. Cells were cultured in DMEM with RANKL and at various concentrations of cordycepin for three days. After osteoclast differentiation, cells were fixed in 4% paraformaldehyde for 10 min at room temperature, and then stained with a TRAP staining solution according to the manufacturer's instructions.
Copyright and License information: ©2018 Su-Bin Yu et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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