Serum cytokine levels were analyzed using BD cytometric bead array (CBA) mouse inflammation kit (BD Pharmingen, San Diego, CA, USA) on a FACS Calibur Cytometer and analyzed using BD FCAP Array software (BD Bioscience). The cytokine kit covered mouse IL-6, IL-10, IL-12p70, TNF, IFNγ, and MCP-1.
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