Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Determination of drug loading and encapsulation efficiency

WH Wenfei Huang
CT Chi Pong Tsui
CT Chak Yin Tang
LG Linxia Gu
request Request a Protocol
ask Ask a question
Favorite

The concentration of vancomycin in the supernatant was determined by measuring its absorbance via UV spectrophotometry at 280.5 nm. A standard curve, which was used to calibrate the relationship between the drug concentration and UV absorbance, was prepared using drug concentrations ranging from 0.35 to 0.0024 mg/ml in 0.5% of PVA. The standard curve of drug concentration in response to UV absorbance was determined to be y = 0.16672 × −0.00421, with a correlation factor R-square of 0.999 in regression analysis. The encapsulation efficiency of the vancomycin in the nanoparticles was determined by identifying the concentration of the non-encapsulated free drug in the supernatant after centrifugation of the nanoparticle suspension at 12,000 rpm for 30 min. The encapsulation efficiency of the drug-loaded nanoparticles was calculated using the following equation:

The drug loading capacity is defined as follows:

Copyright and License information: The Author(s) ©2018
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A