Canine B-cell lymphoma cell lines CLBL-1 (5 replicates), CLBL-1M (5 replicates), GL-1 (1 replicate), and canine T-cell lines CL-1 (2 replicates) and OSW (4 replicates) were cultured routinely in RPMI 1640//20% FBS/2% penicillin-streptomycin (all components from Biochrom AG) medium. All cells were maintained in a humidified incubator at 37 °C and 5% CO2.
Samples from dogs with naturally occurring lymphomas (12 multicentric B-cell lymphomas, one T-cell lymphoma, and three intestinal lymphomas) were collected from 16 patients at the Small Animal Clinic, University of Veterinary Medicine Hannover, Foundation, Germany according to the respective legislation of the state of Lower Saxony, Germany. Four non-neoplastic lymph nodes were used as controls.
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