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Two-hybrid cDNA library screening and yeast two-hybrid (Y2H) assay
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Total RNA was extracted from wild-type anthers during meiosis. Highly purified and intact mRNA was isolated using mRNA Purification Kit (Life technology, NO. 61006). cDNA library construction was performed using Make Your Own Mate & Plate Library System (Clontech NO.630490) according to the manufacturer’s instructions. The Y2H assay was conducted with Yeast Transformation System 2 (Clontech NO.630439). The yeast strain Y2HGOLD was used for library screening and Y2H assays. All primers used were listed in S2 Table.
Copyright and License information: ©2018 Zhang et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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