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After 7 wk, rats were feed-deprived for 5 h before oral gavage with a glucose solution (2 g/kg BW by using 20% glucose; Sigma-Aldrich). Glycemia was measured by using a glucometer (Freestyle) before (0 min) and after (15, 30, 60, 90, and 120 min) glucose administration. Blood samples (∼100 μL) were collected at each time point and centrifuged as described above to obtain serum for insulinemia analysis.
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