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Ventricular myocytes were incubated with 5 μM Di-8-ANEPPS for 15 min at 37°C. Pluronic F-127 (0.05%) (Thermo Fisher) was included in the loading solution to aid the solubilization of the dye following manufacturer’s instructions. Cells were washed three times with extracellular solution, and dye labeled membranes were imaged using confocal microscopy.
Copyright and License information: ©2018 Agarwal, Gratwohl, Cozad, Yang, Clancy and Harvey.
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