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Ethylene biosynthesis was measured as previously described (Leibman-Markus et al., 2017). Leaf disks 0.9 cm in diameter were taken from transiently expressing plants 40 h post-infection. Every five disks were sealed in a 10 mL flask containing 1 ml assay medium (with or without 1 μg/mL EIX) for 4 h at room temperature. Ethylene production was measured by gas chromatography using Varian 3350 equipment.
Copyright and License information: ©2018 Pizarro, Leibman-Markus, Schuster, Bar, Meltz and Avni.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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